产品展厅
产品展厅
储存条件:BSA附件-20℃，其它试剂RT，有效期1年
英文名称:BCA Protein Assay Kit
产品简介
产品简介：
碱性条件下，蛋白将Cu2+还原为Cu+， Cu+与BCA试剂形成紫蓝色的络合物，测定其在562nm处的吸收值，并与标准曲线对比，即可计算待测蛋白的浓度。常用浓度的去垢剂SDS，Triton X-100，Tween不影响检测结果，但受螯合剂(EDTA, EGTA)、还原剂（DTT，巯基乙醇）和脂类的影响。实验中，若发现样品稀释液或裂解液本身背景值较高，可试用Bradford蛋白浓度测定试剂盒。
操作说明：
一. 微孔酶标仪法
1. 配制工作液：根据标准品和样品数量，按50体积BCA试剂加1体积Cu试剂（50:1）配制成BCA工作液，充分混匀(混合时可能会有浑浊，但混匀后就会消失)。BCA工作液室温24小时内稳定。
2. 稀释标准品：取10μL BSA标准品用PBS稀释至100μL（样品一般可用PBS稀释），使终浓度为0.5mg/mL。将标准品按0, 2, 4, 6, 8, 12, 16, 20μL加到96孔板的蛋白标准品孔中，加PBS补足至20μL。
3. 将样品作适当稀释（ 多做几个梯度，如作2倍、4倍、8倍稀释），加20μL到96孔板的样品孔中。由于移液器在取小量样品时误差偏大，标准线前面的点可能不很准确，所以尽可能的让样品点落在标准线1/2后。
4. 各孔加入200μL BCA工作液，37℃放置15-30分钟。用酶标仪测定A562nm，根据标准曲线计算出蛋白浓度。使用温箱孵育时，应注意防止因水分蒸发影响检测结果。
二. 分光光度计法 如没有酶标仪，可用分光光度计在离心管中混匀后加入比色皿中比色。 步骤如下：
1. 配制工作液: 根据标准品和样品数量，按50体积BCA试剂加1体积Cu试剂（50:1）配制成BCA工作液，充分混匀(混合时可能会有浑浊，但混匀后就会消失)。BCA工作液室温24小时内稳定。
2. 稀释标准品：取100μL BSA标准品用PBS稀释至1mL（样品一般可用PBS稀释），使终浓度为0.5mg/mL。
3. 取八支（或者更多）5mL离心管，标上号，按下表加入试剂。
4.37℃放置15-30分钟。用分光光度计测562nm处吸光值，根据标准曲线计算出蛋白浓度。
注意事项：
1. 长期不用时，Cu试剂与PBS稀释液可置于2-8℃保存，如发现细菌污染则应丢弃。BCA试剂在低温条件下出现结晶沉淀时，可37℃温育使其完全溶解, 不影响使用。
2. 样品中若含有较多干扰物质时，请采用Bradford蛋白浓度测定试剂盒。
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
参考文献：
《Effect of 1α, 25-dihydroxyvitamin D3 on the osteogenic differentiation of human periodontal ligament stem cells and the underlying regulatory mechanism》 作者:Yawen Ji，Panpan Zhang，Yixiao Xing，Linglu Jia，Yunpeng Zhang，Tingting Jia，Xuan Wu，Bin Zhao，Xin Xu 期刊:International Journal of Molecular Medicine 影响因子:2.928 PMID:30365053
《Gecko Active Components Regulates Endoplasmic Reticulum Stress to Induce the Apoptosis of KYSE150 Cells through PERK Pathway》 作者:Yimeng Duan, Lengxin Duan, Zeyue Huang, Bingbing Wang, Jiangang Wang 期刊:Journal of Biosciences and Life Sciences 影响因子:0.552 PMID:
《Rhein enhances the cytotoxicity of effector lymphocytes in colon cancer under hypoxic conditions》 作者:Xiangfei Yuan,Wencong Tian,Yang Hua,Lijuan Hu,Jing Yang,Junmuzi Xie,Jiacai Hu,Feng Wang 期刊:Experimental and Therapeutic Medicine 影响因子:1.448 PMID:30542494
《Pro-apoptotic effect of TRAIL-transfected endothelial progenitor cells on glioma cells》 作者:Xin Deng,Wen Zhao,Laijun Song,Wei Ying,Xinbin Guo 期刊:Oncology Letters 影响因子:0.237 PMID:29545899
《Downregulation of transcription factor EB inhibits the growth and metastasis of colorectal carcinomas》 作者:Jin-Zhe Chang,Shu-Dong Chen,Hui Zheng,Hua-Ping Zhang 期刊:European Journal of Inflammation 影响因子:0.733 PMID:
《Dexmedetomidine attenuates the neurotoxicity of propofol toward primary hippocampal neurons in vitro via Erk1/2/CREB/BDNF signaling pathways》 作者:Youbing Tu, Yubing Liang, Yong Xiao, Jing Lv, Ruicong Guan, Fei Xiao, Yubo Xie, and Qiang Xiao 期刊:Drug Design, Development and Therapy 影响因子:3.486 PMID:30858699
《Eicosapentaenoic acid-containing phosphatidylcholine promotes osteogenesis:mechanism of up-regulating Runx2 and ERK-mediated phosphorylation of PPARγ at serine 112》 作者:Lei Mao，Meiling Wang，Yuanyuan Li，Yaxuan Liu，Jingfeng Wang，Changhu Xue 期刊:Journal of Functional Foods 影响因子:3.197 PMID:
《Lipopolysaccharide from Escherichia coli stimulates osteogenic differentiation of human periodontal ligament stem cells through Wnt/β‐catenin–induced TAZ elevation》 作者:Yixiao Xing, Yunpeng Zhang, Linglu Jia, Xin Xu 期刊:Molecular Oral Microbiology 影响因子:2.925 PMID:30387555
《Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway》 作者:Hongyan Gao，Zhe Bo，Qin Wang，Ling Luo，Haiyi Zhu，Yi Ren 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:30553195
《miR‐193a/b‐3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells》 作者:Baoling Ju, Ying Nie, Xufang Yang, Xiaohua Wang, Fujuan Li, Meng Wang, Chuang Wang, Hongjun Zhang 期刊:Journal of Cellular and Molecular Medicine 影响因子:4.658 PMID:30945448
《Cetuximab and Doxorubicin loaded dextran-coated Fe3O4 magnetic nanoparticles as novel targeted nanocarriers for non-small cell lung cancer》 作者:Qinlu Zhang,Qian Liu,Menghan Du,Alphons Vermorken,Yali Cui,Lixia Zhang,Lili Guo,Le Ma,Mingwei Chen 期刊:Journal of Magnetism and Magnetic Materials 影响因子:2.683 PMID:
《HDAC2, but not HDAC1, regulates Kv1.2 expression to mediate neuropathic pain in CCI rats》 作者:Zhihua Li, Yanyan Guo, Xiuhua Ren, Lina Rong, Minjie Huang, Jing Cao, Weidong Zang 期刊:Neuroscience 影响因子:3.244 PMID:31022463
《Overexpression of SENP1 reduces the stemness capacity of osteosarcoma stem cells and increases their sensitivity to HSVtk/GCV》 作者:Fengting Liu， Lili Li， Yanxia Li， Xiaofang Ma， Xiyun Bian， Xiaozhi Liu， Guowen Wang， Dianying Zhang 期刊:International Journal of Oncology 影响因子:3.571 PMID:
《Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture》 作者:Yi-Meng Duan, Ying Jin, Meng-Li Guo, Leng-Xin Duan, and Jian-Gang Wang 期刊:Journal of Cancer 影响因子:3.182 PMID:30087713
《Minor structural modifications to Pracinostat produce big changes in its biological responses》 作者:Rong Jia， Pengju Sun， Yan Zhang， Youjin Ge， Niefang Yu 期刊:Chemical Biology & Drug Design 影响因子:2.256 PMID:30932330
《KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells》 作者:Xu Liu,Xiao-Zhong Guo,Hong-Yu Li,Jiang Chen 期刊:Hepatobiliary & Pancreatic Diseases International 影响因子:1.576 PMID:30948327
《Total flavonoid extract of Epimedium herb increases the peak bone mass of young rats involving enhanced activation of the AC10/cAMP/PKA/CREB pathway》 作者:Hui-Rong Xia, Hui-Ping Ma,Fang-Fang Yang, Yu-Hai Gao,Jian Zhou, Yuan-Yuan Wang, Wen-Yuan Li 期刊:Journal of Ethnopharmacology 影响因子:3.414 PMID:29783019
《MicroRNA?26b acts as an antioncogene and prognostic factor in cervical cancer》 作者:Lihong Wang, Wen Wang, Yuanyuan Wu 期刊:Oncology Letters 影响因子:1.871 PMID:30867779
《Proanthocyanidins Protect against β-Hydroxybutyrate-Induced Oxidative Damage in Bovine Endometrial Cells》 作者:Xi Cheng 1,?OrcID, Shuhua Yang 1,?, Chuang Xu 2, Lanzhi Li 1, Yi Zhang 1, Yang Guo 1, Cai Zhang 3, Peng Li 1,* , Miao Long 1,* OrcID and Jianbin He 4,* 期刊:Molecules 影响因子:3.098 PMID:30678309
《Bufei Jianpi Granules Reduce Quadriceps Muscular Cell Apoptosis by Improving Mitochondrial Function in Rats with Chronic Obstructive Pulmonary Disease》 作者:Jing Mao,1,2 Ya Li,2,3,4,5 Suyun Li,2,3,4,5 Jiansheng Li,2,3,4,5 Yange Tian,2,5 Suxiang Feng,1,2,5 Xuefang Liu,2,5 Qingqing Bian,2,4 Junzi Li,2,4 Yuanyuan Hu,2,4 Lanxi Zhang,2,4 and Huige Ji2, 期刊:Evid Based Complement Alternat Med. 影响因子:6.306 PMID:30723509
《Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation》 作者:YuXiaKangHeLiliJiaoYuanyuanGengDaochuanZhang 期刊:Fish & Shellfish Immunology 影响因子:3.185 PMID:30711491
《Portable Smartphone-based Colorimetric Analyzer with Enhanced Gold Nanoparticles for On-site Tests of Seafood Safety》 作者:Longjie ZHONG, Jiadi SUN, Ying GAN, Shuqi ZHOU, Zijian WAN, Quchao ZOU, Kaiqi SU, Ping WANG 期刊:Analytical Sciences 影响因子:1.355 PMID:30745510
《Immunomodulatory effects of Alhagi honey polysaccharides encapsulated into PLGA nanoparticles》 作者:AdelijiangWusimanabShuwenXuabHaiyuNiabPengfeiGuabZhenguangLiuabYueZhangabTianxinQiuabYuanliangHuabJiaguoLiuabYiWuabDeyunWangabYuLuc 期刊:Carbohydrate Polymers 影响因子:5.158 PMID:30824082
《Lower dosage of aspirin promotes cell growth and osteogenic differentiation in murine bone marrow stromal cells》 作者:MiDu,WanPan,XiaoqiDuan,PishanYang,ShaohuaGe 期刊:Journal of Dental Sciences 影响因子:0.619 PMID:30894990
《Akt and β-catenin contribute to TMZ resistance and EMT of MGMT negative malignant glioma cell line》 作者:Guo-zhong Yi,Ya-wei Liu,Wei Xiang,Hai Wang,Zi-yang Chen,Si-di Xie,Song-tao Qi 期刊:Journal of the Neurological Sciences 影响因子:2.448 PMID:27423571
《Loading icariin on titanium surfaces by phase-transited lysozyme priming and layer-by-layer self-assembly of hyaluronic acid/chitosan to improve surface osteogenesis ability》 作者:Yunjia Song,Aobo Ma,Jia Ning,Xue Zhong,Qian Zhang,Xu Zhang,Guang Hong,Ying Li,and Changyi Li 期刊:International journal of nanomedicine 影响因子:4.37 PMID:30425487
《Vitellogenin2: spermatozoon specificity and immunoprotection in mud crabs》 作者:Ya’nan Yang , Biqi Zheng , Chenchang Bao , Huiyang Huang and Haihui Ye 期刊:Reproduction 影响因子:1.647 PMID:27458256
"《Inactivated Tianjin strain, a novel genotype of Sendai virus, induces apoptosis in HeLa, NCI-H446 and Hep3B cells》 作者:Jun Chen, Han Han, Bin Wang, Liying Shi 期刊:Oncology
Letters 影响因子:1.664 PMID:27347098"
《Neuronic autophagy contributes to p-connexin 43 degradation in hippocampal astrocytes following traumatic brain injury in rats》 作者:Li Qian Sun, Jun Ling Gao, Ying Cui, Man Man Zhao, Xiao Bin Jing, Ran Li, Yan Xia Tian, Jian Zhong Cui, Zhong-Xue Wu 期刊:Molecular Medicine Reports 影响因子:1.922 PMID:25626126
《Protein kinase Cα inhibits myocardin-induced cardiomyocyte hypertrophy through the promotion of myocardin phosphorylation》 作者:Weizong Li, Nan Wang, Man Li, Huiqin Gong, Xinghua Liao, Xiaolong Yang , Tongcun Zhang 期刊:Acta Biochimica et Biophysica Sinica 影响因子:1.807 PMID:26206583
《Effects of Chicory on Serum Uric Acid, Renal Function, and GLUT9 Expression in Hyperuricaemic Rats with Renal Injury and In Vitro Verification with Cells》 作者:Yong-Nan Jin,Zhi-Jian Lin,Bing Zhang, and Yun-Fei Bai 期刊:Evidence-Based Complementary and Alternative Medicine 影响因子:1.722 PMID:30622589
《Sialoglycoprotein isolated from Carassius auratus eggs promotes osteogenesis by stimulating mesenchymal stem cells to commit to osteoblast differentiation》 作者:Lei Mao，Meiling Wang，Xingjun Xi，Yufeng Dai，Na Wang，Jingfeng Wang，Changhu Xue 期刊:Cell and Tissue Research 影响因子:3.36 PMID:30715559
《MicroRNA-378-3p/5p suppresses migration and invasion of oral squamous carcinoma cells by inhibiting KLK4 expression》 作者:Zhi Cui, Shiqun Sun, Qilin Liu, Xuechun Zhou, Siyu Gao, Peixuan Peng, Qianpeng Li 期刊:Biochemistry and Cell Biology 影响因子:2.014 PMID:31265790
《The downregulation of choline kinase alpha inhibits clear cell renal cell carcinoma proliferation and metastasis via the MAPK and PI3K/AKT pathways》 作者:Feng-Shi Zhang, Yang Hong, Zhen-Jie Zhu, Li-Zhe An, Xiao-Bo Huang, Qing-Quan Xu 期刊:Int J Clin Exp Med 影响因子:0.181 PMID:
《miR?210?3p regulates cell growth and affects cisplatin sensitivity in human ovarian cancer cells via targeting E2F3》 作者:Yue Jin， Jun Wei， Shaoting Xu， Fang Guan， Lijun Yin ，Haibin Zhu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:30957179
《RIP3 inhibition protects locomotion function through ameliorating mitochondrial antioxidative capacity after spinal cord injury》 作者:Yang Wang，Jianhang Jiao，Shanyong Zhang，Changjun Zheng，Minfei Wu 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:31146112
《Erythropoietin helix B surface peptide modulates miR-21/Atg12 axis to alleviates cardiomyocyte hypoxia-reoxygenation injury》 作者:Song Huang, Yongluan Lin, Zhanbo Liang, Zhuomin Wu,Yequn Chen and Chang Chen 期刊:American Journal of Translational Research 影响因子:3.266 PMID:31105848
《Examination of Alzheimer's disease by a combination of electrostatic force and mechanical measurement》 作者:W. ZHAO W. CUI S. XU L.‐Z. CHEONG C. SHEN 期刊:Journal of Microscopy 影响因子:1.813 PMID:31038737
《Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the expression of Gadd45b in cell cycle pathway》 作者:Zhongrui Yan, Fengyi Guo, Qing Yuan, Yu Shao, Yedan Zhang, Huiyan Wang, Shaohua Hao and Xue Du 期刊:Stem Cell Research & Therapy 影响因子:4.627 PMID:30606243
《ApoM-S1P Modulates Ox-LDL-Induced Inflammation Through the PI3K/Akt Signaling Pathway in HUVECs》 作者:Zhi Zheng,Yongzhi Zeng,Xiao Zhu,Ying Tan,Yi Li,Qian Li,Guanghui Yi 期刊:Inflammation 影响因子:2.939 PMID:30377890
《The expression of melatonin receptors MT1 and MT2 is regulated by E2 in sheep oviduct》 作者:Jun-jie Hu，Long-fei Xiao，Liang-li Song，Wen-bo Ge，Hong-wei Duan，Yuting Jiang 期刊:General and Comparative Endocrinology 影响因子:2.445 PMID:30849411
《E2F1 mediates the downregulation of POLD1 in replicative senescence》 作者:Shichao Gao,Qiao Song,Jing Liu,Xiaomin Zhang,Xunming Ji,Peichang Wang 期刊:Cellular and Molecular Life Sciences 影响因子:7.014 PMID:30895337
《Synergistic protection of matrine and lycopene against lipopolysaccharide?induced acute lung injury in mice》 作者:Wu?Wei Li， Tao?Yuan Wang ，Bo Cao， Bin Liu， Yu?Mei Rong ，Juan?Juan Wang ，Fei Wei ，Lu?Qing Wei， Hong Chen ，Yan?Xia Liu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:31180535
《A thermosensitive RGD-modified hydroxybutyl chitosan hydrogel as a 3D scaffold for BMSCs culture on keloid treatment》 作者:Congcong，Qu，ZixianBao，Xin，Zhang，Zhiguo，Wang，Jizhen，Ren，Zhongzheng，Zhou，Meiping Tian，Xiaojie Cheng，Xiguang Chen，Chao Feng 期刊:International Journal of Biological Macromolecules 影响因子:4.784 PMID:30529347
《Protective effects of higenamine combined with [6]-gingerol against doxorubicin-induced mitochondrial dysfunction and toxicity in H9c2 cells and potential mechanisms》 作者:Jianxia Wen，Jian Wang，Pengyan Li，Ruilin Wang，Jiabo Wang，Xuelin Zhou，Lu Zhang，Haotian Li，Shizhang Wei，Huadan Cai，Yanling Zhao 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:31028997
《Expression of inositol?requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium?induced mouse colonic tumors》 作者:Faliang Dai， Shizhen Dong， Zhenhua Rong， Qingxia Xuan， Pan Chen ，Menglu Chen， Yonggang Fan， Qiang Gao 期刊:Experimental and Therapeutic Medicine 影响因子:1.448 PMID:30603916
《C14orf159 suppresses gastric cancer cells’ invasion and proliferation by inactivating ERK signaling》 作者:Yan-Mei Zhu,Qiang Li, Xiao-Zhuo Gao, Xiao Meng, Li-Li Sun,Yu Shi,En-Tian Lu and Yong Zhang 期刊:Cancer Management and Research 影响因子:2.243 PMID:30863180
《Unfolded Protein Response Differentially Regulates TLR4-Induced Cytokine Expression in Distinct Macrophage Populations》 作者:Lei Zhang, Paul G. Pavicic Jr., Shyamasree Datta, Qiaoling Song, Xiaohan Xu, Wei Wei, Fan Su, Patricia A. Rayman, Chenyang Zhao and Thomas Hamilton 期刊:Frontiers in Immunology 影响因子:4.716 PMID:31293572
《miR?27b?3p and miR?607 cooperatively regulate BLM gene expression by directly targeting the 3'?UTR in PC3 cells》 作者:Yinglian Chen, Jiafu Zhao ,Zhiqiang Duan ,Ting Gong ,Wei Chen, Sainan Wang ,Houqiang Xu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:30957187
《Toll-like receptor 4 is necessary for glucose-dependent glucagon-like peptide-1 secretion in male mice》 作者:Lijuan Wang,Xiandong Zhan,Zhenhui Wang,Jing Ma,Xiaotong Chang,Xiaobo Zhu 期刊:Biochemical and Biophysical Research Communications 影响因子:2.705 PMID:30661789
《Homeobox A10 promotes the proliferation and invasion of bladder cancer cells via regulation of matrix metalloproteinase?3》 作者:Chunlei Liu, Mingzhu Ge, Jun Ma, Yanhui Zhang, Yanhui Zhao, Tao Cui 期刊:Oncology Letters 影响因子:1.871 PMID:31289471
《Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways》 作者:Kunxiao Zhao,Ying Li, Ziqiang Wang, Ning Han, and Ying Wang 期刊:BioMed Research International 影响因子:2.197 PMID:31275971
《Glycyrrhetinic Acid Improves Insulin-Response Pathway by Regulating the Balance between the Ras/MAPK and PI3K/Akt Pathways》 作者:Yuan Zhang, Shengnan Yang, Man Zhang, Zhihua Wang, Xin He, Yuanyuan Hou and Gang Bai 期刊:Nutrients 影响因子:4.171 PMID:30871060
《A novel ELISA for the detection of active form of plasminogen activator inhibitor-1 based on a highly specific trapping agent》 作者:Le Shang，Guangpu Xue，Lihu Gong，Yaxin Zhang，Shuangzhou Peng，Cai Yuan，Mingdong Huang 期刊:Analytica Chimica Acta 影响因子:5.256 PMID:30712574
