产品展厅
产品展厅
P1200-25T | P1200-50T | 保存条件 | |
30%制胶液(29:1) | 100mL | 100mL ×2 | 4℃，避光 |
1.5mol/LTris(pH8.8) | 100mL | 100mL×2 | 常温 |
1.0mol/LTris(pH6.8) | 30mL | 60mL | 常温 |
PAGE胶凝固剂 | 1g | 2g | 干粉4℃；溶液-20℃ |
10%SDS | 5mL | 10mL | 常温 |
PAGE胶促凝剂 | 0.8mL | 1.5mL | 4℃，避光 |
产品简介：
本公司生产的SDS-PAGE凝胶制备试剂盒是按照经典方法配制而成。本试剂盒提供了配制SDS-PAGE凝胶所需的各种试剂，用户只需自备制胶器具和蒸馏水，即可配制PAGE胶。本试剂盒可配制不同规格、不同聚合度的PAGE胶(即聚丙烯酰胺凝胶) 约25/50块。
注意事项：
1. PAGE胶促凝剂和10%PAGE胶凝固剂*加，在加入前需混匀前面所加试剂。10%PAGE胶凝固剂溶液在4℃有效期为一周； PAGE胶促凝剂易挥发，使用后请盖紧瓶盖。在常温下胶30分钟内可以凝固，如温度过低，可放37℃温箱凝固。灌完分离胶后，轻轻的加1mL ddH2O封上层，胶凝固后可见到分界线。灌浓缩胶前，先倾去水层，再用吸水纸吸干。浓缩胶灌好后立刻插入梳子。浓缩胶凝固后，放入电泳液中(让电泳液漫过加样孔)，轻轻的拨出梳子，可防加样孔变形。
2. 配制量可按上表等比加减。如果所用胶浓度与上面不同，可自行调整，主要是调整30% 制胶液的量（需要浓度×总体积/30%），*用水补足总体积。
配胶说明：
1. 先在PAGE胶凝固剂干粉中加入蒸馏水或去离子水(每克PAGE胶凝固剂需加水10mL)配置成10%溶液，将溶液分装成小体积后冻存于-20℃，制备凝胶时融化后使用。4℃保存有效期为一周。检测SDS溶液是否澄清，若出现絮状物或沉淀可放37℃片刻至溶解，不影响效果。
2. 根据目标蛋白分子量大小，选取凝胶浓度，按下表配制。先配分离胶，再配浓缩胶。
分离胶12% | 分离胶8% | <span font-size:12pt;"="" style="box-sizing: border-box">浓缩胶5% | |||
总体积 | 10mL | 10mL | 10mL | 10mL | 5mL |
30% 制胶液(29:1) | 5mL | 4mL | 3.3mL | 2.7mL | 0.83mL |
1M Tris-HCl (PH6.8) | 0 | 0 | 0 | 0 | 0.625mL |
1.5M Tris-HCl(PH8.8) | 2.5mL | 2.5mL | 2.5mL | 2.5mL | 0 |
10%SDS | 100μL | 100μL | 100μL | 100μL | 50μL |
10%PAGE胶凝固剂 | 100μL | 100μL | 100μL | 100μL | 75μL |
PAGE胶促凝剂 | 10μL | 10μL | 10μL | 10μL | 7.5μL |
ddH2O | 2.3mL | 3.3mL | 4.0mL | 4.6mL | 3.42mL |
*分离范围 | 10-40kD | 12-60 kD | 20-80 kD | 30-90kD | — |
参考文献：
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《Long noncoding RNA ENST00000413528 sponges microRNA‐593‐5p to modulate human glioma growth via polo‐like kinase 1》 作者:Ren Zhang，Ruo‐Lun Wei，Wei Du，Li‐Wei Zhang，Tao Du，Ya‐Dong Geng，Xin‐ting Wei 期刊:CNS Neuroscience & Therapeutics 影响因子:4.458 PMID:30924320
《Disparate expression of autophagy in corneas of C57BL/6 mice and BALB/c mice after Aspergillus fumigatus infection》 作者:Chen-Yu Li, Cui Li, Hui Li, Gui-Qiu Zhao, Jing Lin, Qian Wang, Xu-Dong Peng, Qiang Xu, Guo-Qiang Zhu, and Jia-Qian Jiang 期刊:International Journal of Ophthalmology 影响因子:1.189 PMID:31131226
《Maresin1 regulates neutrophil recruitment and IL-10 expression in Aspergillus fumigatus keratitis》 作者:Qing Tang,Chengye Che,Jing Lin,Hong He,Wenyi Zhao,Leyu Lv,Guiqiu Zhao 期刊:International Immunopharmacology 影响因子:1.638 PMID:30690344
《Characterization, Expression Profiling, and Functional Analyses of a 4CL-Like Gene of Populus trichocarpa》 作者:Hui Wei, Chen Xu, Ali Movahedi, Weibo Sun and Qiang Zhuge 期刊:Processes 影响因子:2.973 PMID:
《Total flavonoid extract of Epimedium herb increases the peak bone mass of young rats involving enhanced activation of the AC10/cAMP/PKA/CREB pathway》 作者:Hui-Rong Xia, Hui-Ping Ma,Fang-Fang Yang, Yu-Hai Gao,Jian Zhou, Yuan-Yuan Wang, Wen-Yuan Li 期刊:Journal of Ethnopharmacology 影响因子:3.414 PMID:29783019
《Proanthocyanidins Protect against β-Hydroxybutyrate-Induced Oxidative Damage in Bovine Endometrial Cells》 作者:Xi Cheng 1,?OrcID, Shuhua Yang 1,?, Chuang Xu 2, Lanzhi Li 1, Yi Zhang 1, Yang Guo 1, Cai Zhang 3, Peng Li 1,* , Miao Long 1,* OrcID and Jianbin He 5,* 期刊:Molecules 影响因子:3.098 PMID:30678309
《High Spy1 expression predicts poor prognosis in colorectal cancer》 作者:Qin Jin, Gang Liu, Luri Bao,Yuzhen Ma,Huidong Qi,ZhizhongYun,Yanfeng Dai, and Shu Zhang 期刊:Cancer Management and Research 影响因子:3.702 PMID:30147372
《MicroRNA?19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor?κB》 作者:Fan Yang, Hongjian Wang ,Zhenyu Jiang, Anxiang Hu ,Lisha Chu, Yiling Sun, Junqing Han 期刊:Molecular Medicine Reports 影响因子:1.922 PMID:26239140
《Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model》 作者:Lina Liu, Guodong Lv, Conghua Ning, Ye Yang, Jun Zhu 期刊:Experimental and Therapeutic Medicine 影响因子:1.41 PMID:27284312
"《Purification of the insecticidal Cry2Ad protein
from a Bt-isolated BRC-HZP10 strain and
its toxin assay to the diamondback moth,
Plutella xylostella (L.)》 作者:J.Y. Liao, Y.Q. Gao, Q.Y. Wu, Y.C. Zhu and M.S. You 期刊:Genetics and Molecular Research 影响因子:0.58 PMID:26214446"
《A deuterohemin peptide protects a transgenic Caenorhabditis elegans model of Alzheimer’s disease by inhibiting Aβ1–42 aggregation》 作者:Jia Xu,Ye Yuan,Ruining Zhang,Yanhui Song,Tianzhuo Sui,Jiaqi Wang,Chonghan Wang,Yujia Chen,Shuwen Guan,Liping Wang 期刊:Bioorganic Chemistry 影响因子:3.926 PMID:30428413
《Plant-Based Nanoparticles Prepared from Proteins and Phospholipids Consisting of a Core–Multilayer-Shell Structure: Fabrication, Stability, and Foamability》 作者:Xing Chen，Yan Chen，Liqiang Zou，Xingcai Zhang，Yuqing Dong，Jizhou Tang，David Julian McClements，Wei Liu 期刊:Journal of Agricultural and Food Chemistry 影响因子:3.571 PMID:31117503
《Effects of ultrasonics combined with far infrared or microwave thawing on protein denaturation and moisture migration of Sciaenops ocellatus (red drum)》 作者:Luyun Cai，Wendi Zhang，Ailing Cao，Minjie Cao，Jianrong Li 期刊:Ultrasonics Sonochemistry 影响因子:7.279 PMID:31084796
《Synergistic protection of matrine and lycopene against lipopolysaccharide?induced acute lung injury in mice》 作者:Wu?Wei Li， Tao?Yuan Wang ，Bo Cao， Bin Liu， Yu?Mei Rong ，Juan?Juan Wang ，Fei Wei ，Lu?Qing Wei， Hong Chen ，Yan?Xia Liu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:31180535
