宝子们！科研大揭秘来啦！上海交通大学医学院附属瑞金医院的研究发现，维生素 C 可不只是普通的营养素，它能直接修饰蛋白质赖氨酸残基，特别是让信号转导和转录激活因子 1（STAT1） 的赖氨酸 298 位点发生 vitcylation，增强干扰素信号转导，从而激活咱们身体的抗肿瘤免疫反应，这对提升免疫力简直太关键啦！

  维生素C  

维生素C（也称为抗坏血酸）是一种水溶性维生素，具有抗氧化特性，在人体多种生理过程中发挥关键作用，如参与胶原蛋白合成、促进铁吸收以及增强免疫功能等。

其实，维生素家族里的其他成员也都各有神通，在守护我们健康这件事上，一个都没落下。基于这一前沿成果，北京索莱宝携旗下丰富的维生素家族产品，邀您一同拥抱健康新高度。

实验案例一：

I  F  33.8990

文献

Sun L, Zhang Y, Chen G, Ji Y, Ma Q, Qiao X, Wu S, Zhou L, Bu J, Zhu X, Zhang X, Jiang X, Liu C, Li X, Liu Y, Yang Y, Liu C. 

Targeting SOST using a small-molecule compound retards breast cancer bone metastasis. 

Mol Cancer. 2022 Dec 29;21(1):228.

DOI:10.1186/s12943-022-01697-4. 

PMID:36581888.

PMCID: PMC9798707.

方法 诱导小鼠MC3T3-E1 cells成骨分化；50 μg/mL L-ascorbic acid

Sun L, et al. doi: 10.1186/s12943-022-01697-4.

实验案例二：

 I  F  36.3009

文献

Ren J, Zhang Z, Geng S, Cheng Y, Han H, Fan Z, Dai W, Zhang H, Wang X, Zhang Q, He B.

Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling.

Nanomicro Lett. 2024 Feb 1;16(1):103.

DOI:10.1007/s40820-023-01313-0.

PMID:38300384.

PMCID: PMC10834923.

方法  

Mouse macrophage cell line J774A.1 ：

制备了与辣根过氧化物酶（HRP）偶联的聚乳酸 - 羟基乙酸共聚物纳米颗粒作为模型（HRP (+)-PNPs），以评估纳米药物在巨噬细胞中的递送分子机制。
The reaction was quenched after 1 min by the addition of a 100 X "quencher solution," comprising 1 M sodium azide, 1 M sodium ascorbate, and 500 mM Trolox in PBS.

Ren J, et al. doi: 10.1007/s40820-023-01313-0.

实验案例三：

I  F  25.9002

文献

Wang P, Zhang L, Chen S, Li R, Liu P, Li X, Luo H, Huo Y, Zhang Z, Cai Y, Liu X, Huang J, Zhou G, Sun Z, Ding S, Shi J, Zhou Z, Yuan R, Liu L, Wu S, Wang G. 

ANT2 functions as a translocon for mitochondrial cross-membrane translocation of RNAs.

Cell Res. 2024 Jul;34(7):504-521.

DOI:10.1038/s41422-024-00978-5. 

Epub 2024 May 29.

PMID:38811766.

PMCID:PMC11217343.

方法

合成蛋白质的叠氮高丙氨酸（AHA）标记：

5mM sodium ascorbate (Solarbio) for 2 h

Experimental procedures for AHA labeling of synthesized proteins were adapted from a published protocol. The sample was incubated at 95 °C for 10 min. Click reaction was then performed after the samples were cooled to 37 °C in a reaction system containing 50 μMAcety-lene-PEG4-Biotin, 0.1 mM GuSO4,0.5 mM THPTA , and

5mM sodium ascorbate (Solarbio) for 2 h. The reaction was terminated with 1 mM EDTA. The labeled proteins were resolved by SDS–PAGE and detected with Biotin detection reagents.

Wang P, et al. doi: 10.1038/s41422-024-00978-5.

Wang P, et al. doi: 10.1038/s41422-024-00978-5.

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